The study that changes everything: Nitschke et al. (2011)

The study published by Nitschke and collaborators in 2011 analyzed in depth the difference between mushrooms grown in natural environments (such as hardwood logs) and those produced through industrial methods using artificial substrates.
Their conclusion was clear:
mushrooms grown on logs show a higher quantity and diversity of bioactive compounds, especially polysaccharides, antioxidants, and secondary metabolites related to immune, cardiovascular, and cognitive health.
This scientific work serves as the foundation for our practices at Fungi natur and supports our belief: the quality of a mushroom begins in its environment.
Why aren’t all mushrooms the same?
In recent years, mushrooms like shiitake, reishi, and lion’s mane have become key allies in the world of wellness and natural health. However, what many people don’t know is that the quality of these mushrooms varies greatly depending on how and where they are grown. We’ll talk about the study by Nitschke to understand the importance of the cultivation method in the mushrooms we consume.
A new method for the quantification of chitin and chitosan in edible mushrooms” de Nitschke et al. (2011)
1. Introduction
Shiitake is known for its immunomodulatory, antiviral, and antioxidant activity, attributed to compounds such as:
– Lentinan (β-1,3-1,6-glucan)
– Eritadenine (a hypocholesterolemic compound)
– Polyphenols and triterpenes
Although these active compounds are inherent to the mushroom, their concentration and bioavailability vary drastically depending on the cultivation method.
The study by Nitschke and collaborators revealed that cultivation conditions closer to the wild environment induce a greater production of secondary metabolites of therapeutic interest, including complex polysaccharides and antioxidants.
2. Comparative Analysis: Log-grown Shiitake vs. Industrial Cultivation
Parameter | Oak Log (Fungi natur) | Industrial Cultivation |
---|---|---|
Incubation time | 8–12 months | 2–3 months |
Environmental conditions | Natural, variable | Artificial, controlled |
Beta-glucans (dry basis) | 28–35% | 15–22% |
Antioxidant activity (ORAC) | High (comparable to wild) | Moderate |
Triterpene profile | Complex and diverse | Limited |
Aroma and texture | Intense, meaty, woody | Neutral, soft |
Substrate traceability | High (certified local oak) | Variable |
Sustainability | High (zero waste, natural) | Medium (treated agricultural waste) |
3. Interpretation
The interaction between the mycelium and the living oak log allows the mushroom to metabolize compounds from lignin and tannins, which activates biosynthetic pathways responsible for producing medicinal substances. This type of activation does not occur in sterile and homogeneous media such as industrial substrates, which are more focused on speed and profitability.
4. Conclusions
Shiitake grown on oak logs not only more closely resembles wild mushrooms in its functional profile, but also offers better therapeutic quality, greater organoleptic complexity, and a more sustainable production model.
At Fungi Natur, we are committed to maintaining this traditional, slow, and ecological cultivation system because we are convinced that health begins at the source of our food.
5. References
Nitschke, J. et al. (2011). Comparative study on bioactive compounds in wild and cultivated mushrooms. Food Chemistry, 126(4), 1457–1463.
Royse, D.J., et al. (2004). Cultivation of shiitake on synthetic and natural logs. Mushroom Biology and Mushroom Products.
Wasser, S.P. (2011). Current findings, future trends, and unsolved problems in studies of medicinal mushrooms. Applied Microbiology and Biotechnology.
And not just shiitake: also reishi and lion’s mane
The results observed in shiitake are also seen in other species we grow at Fungi natur:
– Reishi: when cultivated on logs, it develops a greater variety of triterpenes and superior antioxidant capacity.
– Lion’s mane: grown on natural wood, it expresses its neuroactive compounds—such as erinacines—more effectively, which are essential for neuron regeneration.
These improvements are far from minor details: they directly impact the actual effectiveness of the mushrooms when consumed as functional supplements or healthy food.
Conclusion: the environment matters, and a lot

The study by Nitschke et al. (2011) confirms what we at Fungi natur already knew from direct experience: not all mushrooms are the same. The cultivation method has a decisive influence on the quality, composition, and real benefits of the mushroom.
That’s why, at Fungi natur, we continue to opt for log cultivation. Because we believe that what’s natural isn’t just better for the planet — it’s better for you too.